Anti-PLCG2(Tyr1217) (Polyclonal), ALEXA Fluor 594
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Polyclonals and antibodies
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Alexa conjugate 1
Alexa Fluor,ALEXA FLUOR® 594
For facs or microscopy Alexa 1 conjugate.
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
PLCG2(Tyr1217) Polyclonal Antibody
PLCG2phospho Y1217; PLC Î³2Tyr1217; PLC gamma 2/PLCG2Tyr1217; PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 18.104.22.168; Phosphoinositide phospholipase C; Phospholipase C gamma 2; Phospholipase C, gamma 2 phosphatidylinositol specic; PLCG2_HUMAN.
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Purified by Protein A.
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
KLH conjugated synthetic phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr1217
1ug per 1ul
This antibody reacts specifically with PLCG2(Tyr1217)
Rabbit (Oryctolagus cuniculus)
ALEXA FLUOR® 594
Conjugated Primary Antibody